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科研队伍
研究室
林木生物技术研究室

研究室介绍

本研究室成立于2002年,主要从事林木生物技术和谷子分子育种研究。重点研究内容包括林木细胞壁合成机理和杨树材性改良基因工程育种、谷子种质资源评价保存和生物技术育种。团队现有正高级职称研究人员2名,副高级职称研究人员3名,中级职称研究人员2名,以及多名流动研究人员。研究室先后承担了国家科技部十五到十四五国家重点研发课题10余项,国家自然科学基金项目7项,以及国家转基因重大专项、国家林业局948项目、北京市科委重大转基因专项、现代农业产业技术体系北京市创新团队岗位专家等项目。发表高水平文章20余篇,获得国家发明专利10余项。

人员构成

魏建华王宏芝姚磊张杰伟丁莉萍陈亚娟郑林

成果展示

近期主要项目


[1]国家自然科学基金 “杨树保卫细胞壁力学特性的分子调控机制解析” 面上项目(32271901),2023-2026;
[2]十四五国家重点研发计划课题“林木单倍体高效诱导和加倍技术”(2021YFD2200203)子课题,2021-2026;
[3]北京市农业农村局“现代农业产业技术体系北京市创新团队”岗位专家,2022-2026;
[4]国家自然科学基金 “杨树细胞壁合成基因调控根蘖苗发生的分子机制研究” 面上项目(32171736),2022-2025;
[5]国家重点研发计划“谷子品质育种的分子标记开发”(2019YFD1000703)子课题,2019-2022;
[6]转基因生物新品种培育国家科技重大专项“转基因杨树新品种培育及产业化研究”(2018ZX08020002)子课题,2018-2020;
[7]国家自然科学基金“杨树保卫细胞壁合成调控的分子机制”面上项目(31770708),2018-2021;
[8]国家自然科学基金“基于转录组分析的杨树胚性愈伤组织形成的关键基因发掘及功能分析”青年基金(31800567),2019-2021;
[9]国家重点研发计划课题“林木木质部分化调控因子鉴定及功能解析”(2016YFD0600104)子课题,2016-2020;
[10]北京市农委推广项目“谷子省工轻简栽培技术与配套新品种在京郊的示范推广”,2018-2019。


科研论文

[1]Deng, Y., Zhang, H., Wang, H., Xing, G., Lei, B., Kuang, Z., Zhao, Y., Li, C., Dai, S., Yang, X., et al. The Construction and Exploration of a Comprehensive MicroRNA Centered Regulatory Network in Foxtail Millet (Setaria italica L.). Front Plant Sci, 2022, 13, 848474.
[2]Li, C., Ma, J., Wang, G., Li, H., Wang, H., Wang, G., Jiang, Y., Liu, Y., Liu, G., Liu, G., et al. Exploring the SiCCT Gene Family and Its Role in Heading Date in Foxtail Millet. Front Plant Sci, 2022, 13, 863298.
[3]Li, C., Wang, G., Li, H., Wang, G., Ma, J., Zhao, X., Huo, L., Zhang, L., Jiang, Y., Zhang, J., et al. High-depth resequencing of 312 accessions reveals the local adaptation of foxtail millet. Theor Appl Genet, 2021, 134, 1303-1317.
[4]Zheng L, Yang J, Chen Y, Ding L, Wei J*, Wang H*: An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar. BMC Plant Biology, 2021, 21:54
[5]Ding, L., Chen, Y., Ma, Y., Wang, H*., and Wei, J*. Effective reduction in chimeric mutants of poplar trees produced by CRISPR/Cas9 through a second round of shoot regeneration. Plant Biotechnology Reports, 2020, 14, 549-558.
[6]Lin Zheng#, Yajuan Chen#, Dong Ding, Ying Zhou, Liping Ding, Jianhua Wei*, Hongzhi Wang*, Endoplasmic reticulum–localized UBC34 interaction with lignin repressors MYB221 and MYB156 regulates the transactivity of the transcription factors in Populus tomentosa. BMC Plant Biology, 2019, DOI: 10.1186/s12870-019-1697-y.
[7]Ding LP, Chen YJ, Wang HZ, Wei JH*. Efficacy of Cry1Ac protein against gypsy moth and fall webworm in transgenic poplar (Populus davidiana × Populus bolleana) by bioassay. Canadian Journal of Plant Science, 2018, 98(4): 844-850.
[8]Ding LP, Chen YJ, Wei XL, Ni M, Zhang JW, Wang HZ, Zhu Z, Wei JH*. Laboratory evaluation of transgenic Populus davidiana × Populus bolleana expressing Cry1Ac+SCK, Cry1Ah3, and Cry9Aa3 genes against gypsy moth and fall webworm, PLOS ONE, 2017, 12(6): 1-12.
[9]Wang Y, Chen Y, Ding L, Zhang J, Wei J*, Wang H*. Validation of Reference Genes for Gene Expression by Quantitative Real-Time RT-PCR in Stem Segments Spanning Primary to Secondary Growth in Populus tomentosa. PLoS One, 2016, 11(6): e0157370.
[10]Jiewei Zhang, Keke Xia, Youming Yang, Hailian Yang*, Overexpression of Arabidopsis phosphoinositide-specific phospholipase C5 induces leaf senescence. Plant Cell Tiss Organ Cult, 2015, 120(2): 585-595.
[11]Jiewei Zhang, Zhongbao Zhang, Dan Zhu, Yang Guan, Dianyi Shi, Yajuan Chen, Ruifen Li, Hongzhi Wang*, Jianhua Wei*, Expression and initial characterization of a Phosphoinositide-specific phospholipase C from Populus tomentosa. J. Plant Biochem. Biotechnol, 2014, 24:338-346
[12]Hong-Zhi Wang#, Ying-Xi Xue#, Ya-Juan Chen, Rui-Fen Li, Jian-hua Wei*, Lignin modification improves the biofuel production potential in transgenic Populus tomentosa, Industrial crops and products, 2012, 37(1):170-177.
[13]Wei Jianhua, Wang Yanzhen, Wang Hongzhi, Li Ruifen, Lin Nan, Ma Rongcai,Song Yanru*,Pulping performance of transgenic poplar with depressed Caffeoyl-CoA O-methyltransferase, Chinese Science Bulletin, 2008,53(22): 3553-3558
[14]WANG Hongzhi, MA Rongcai, LI Ruifen, WANG Guoying, WEI Jianhua*, Virus-induced silencing of a tobacco deoxyhypusine synthase gene, Chinese Science Bulletin, 2005, 50(23):2707-2713.
[15]JIA Caihong, ZHAO Huayan, WANG Hongzhi, XING Zhifeng, DU Kejiu, SONG Yanru, WEI Jianhua*, Obtaining the transgenic poplars with low lignin content through down-regulation of 4CL. Chinese Science Bulletin, 2004, 49(9): 905-909.


专利

[1]陈亚娟,魏建华,王宏芝,丁丽萍,姚磊,张杰伟.一种杨树NAC基因启动子及其应用。专利号:ZL 2018 1 0146879.9。
[2]丁莉萍, 魏建华, 王宏芝, 李瑞芬, 张杰伟, 陈亚娟, 张中保, 黄义. 一种可稳定自发光的重组载体的构建方法及其应用。专利号:ZL201510128902.8。
[3]丁莉萍, 王宏芝, 李瑞芬, 张杰伟, 陈亚娟, 魏建华,一套提高根癌农杆菌介导的杨树遗传转化效率的方法。专利号:ZL201510018359.6。
[4]张杰伟, 魏建华, 王宏芝, 李瑞芬, 张中保, 陈亚娟, 管阳, 朱丹,杨树蛋白PtoeIF5A1及其编码基因在调控植物生长中的应用。专利号:ZL201310028790.X。
[5]张杰伟, 魏建华, 王宏芝, 李瑞芬, 张中保, 陈亚娟, 丁莉萍,杨树耐盐基因PtoeIF5A1的应用。专利号:ZL201310700593.8。
[6]王宏芝,魏建华,李云伏,李瑞芬,陈亚娟,张中保,张杰伟,一种提高植物中纤维生物质能源利用效率的方法。专利号:ZL201110359373.4。
[7]王宏芝,魏建华,王彦珍,李瑞芬,马荣才,脱氧羟腐胺赖氨酸合酶编码基因及其反义核苷酸序列。专利号:ZL200810119993.9。
[8]魏建华,宋艳茹,王宏芝,赵华燕,一种调控毛白杨木质素的方法。专利号:ZL2003160093.X。